Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Tylenol, Autism, And The Glutathione Connection OC Integrative Medicine Why you should avoid REGULAR acetaminophen use for babies and pregnancy 3 Little Plums How Keeping Glutathione Levels Up Can Benefit Your Health & Your Vitamin D Status GrassrootsHealth Ferroptosis as a therapeutic target in glioblastoma: Mechanisms and emerging strategies: Molecular Therapy Nucleic Acids