Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Deubiquitinases Maintain Protein Homeostasis and Survival of Cancer Cells upon Glutathione Depletion: Cell Metabolism Identification of cytotoxic, glutathione reactive moieties inducing accumulation of reactive oxygen species via glutathione depletion ScienceDirect How Glutathione is Depleted from the Body Suzy Cohen, RPh offers natural remedies to help you feel better now! Glutathione, often dubbed the master antioxidant plays a crucial role in detoxification, immune function, and protecting our cells from oxidative stress. Here are some signs that your levels might be low: Persistent